Detection of pathogens by next-generation sequencing in horses
Culture and Next-generation sequencing could be used in tandem to offer valuable information in equine reproductive health
Fungal and bacterial culture is currently the primary method for pathogen detection and identification. Next-generation sequencing is a powerful method for detecting and identifying the presence of microbial DNA in samples. The authors evaluated the correlation between fungal and bacterial culture with next-generation sequencing in equine uterine samples.
Fungal cultures (n=63) were evaluated based on their culture results. In culture positive (n=16) samples, next-generation sequencing identified the same organism in 10 samples (62.5%), 5 samples did not identify fungal agents, and 1 sample identified other species of fungal agents. In no growth samples (n=42), next-generation sequencing did not identify fungal ...
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