Serum protein electrophoretic profile changes in West Nile virus infected horses

Serum protein electrophoresis (SPE) is widely used to evaluate protein changes associated with several pathologies, aiming to assist clinical diagnosis. This study determines, for the first time, SPE profile changes in West Nile virus-naturally infected horses. Sixty horses with different clinical and infection status (encephalitis, asymptomatic infection, and immunologically naïve) were included. Total protein concentrations were determined via an automated biuret method and SPE was performed using a cellulose acetate membrane-based automated system. Statistical analysis revealed significantly higher total protein between both infected horse groups (encephalitis, asymptomatic) and naïve controls. Horses with encephalitis had higher globulin and α2-globulin levels than the other ...

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